Carbonyl reductase mutant and application thereof

Carbonyl reductase mutant and application thereof

The invention discloses a carbonyl reductase mutant and application thereof. The reductase mutant disclosed by the invention is prepared by directional mutation of an amino acid sequence as shown in SEQ ID NO: 1. The mutant disclosed by the invention is good in catalytic activity. The e.e. Of partia...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: WANG PING, XIE CHENGFU, MENG XIAO, ZHU LU, ZHANG QING, ZHU JINGYANG, SHI FENG
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention discloses a carbonyl reductase mutant and application thereof. The reductase mutant disclosed by the invention is prepared by directional mutation of an amino acid sequence as shown in SEQ ID NO: 1. The mutant disclosed by the invention is good in catalytic activity. The e.e. Of partial mutants is greater than or equal to 98% and/or the conversion rate is greater than or equal to 95%. 本发明公开了一种羰基还原酶突变体及其应用。本发明的还原酶突变体为如SEQ ID NO:1所示的氨基酸序列定向突变制备得到。本发明突变体的催化活性好。本发明的部分突变体的e.e.≥98％和/或转化率≥95％。