Dental pulp mesenchymal stem cell culture method

The invention provides a culture method of mesenchymal stem cells. The culture method comprises the following steps: adding a mixture of an inonotus obliquus extract and a ginkgo leaf extract into a culture medium; the mesenchymal stem cells are dental pulp mesenchymal stem cells. The inonotus obliquus disclosed by the invention is extremely wide in source, high in safety and easy to obtain; when the mixture of the inonotus obliquus extract and the ginkgo biloba extract is used for culturing the dental pulp stem cells, the cell proliferation capacity can be improved, the culture time is shortened, the time cost is reduced, and the cell activity can be improved; differentiation of the dental pulp stem cells is not induced, and the purity of the dental pulp stem cells is guaranteed. 本发明提供一种间充质干细胞的培养方法，包括向培养基中加入桦褐孔菌提取物和银杏叶提取物的混合物；所述的间充质干细胞为牙髓间充质干细胞。本发明的桦褐孔菌来源极其广泛，安全性高，易获得；桦褐孔菌提取物及银杏提取物混合物培养牙髓干细胞，可以提高细胞增殖能力，缩短培养时间，减少时间成本，同时可以提高细胞活性；且不会诱导牙髓干细胞分化，保证牙髓干细胞的纯度。