Application of NtNRAMP2 protein, method for improving sensitivity of tobacco to cadmium stress and method for obtaining cadmium stress resistant tobacco plant

The invention belongs to the technical field of gene engineering, and particularly relates to application of NtNRAMP2 protein, a method for improving cadmium stress tolerance of tobacco and a method for obtaining a cadmium stress-tolerant tobacco plant. The invention provides application of NtNRAMP2 protein in creating cadmium stress resistant tobacco or breeding cadmium resistant tobacco. The amino acid sequence of the NtNRAMP2 protein is as shown in SEQ ID NO: 2. The NtNRAMP2 protein disclosed by the invention is encoded and synthesized by an NtNRAMP2 gene, and transgenosis and functional identification prove that the NtNRAMP2 natural resistance giant cell-philic protein synthesized by the NtNRAMP2 gene disclosed by the invention is closely related to tobacco cadmium absorption regulation and control. 本发明属于基因工程技术领域，具体涉及NtNRAMP2蛋白的应用、一种提高烟草耐镉胁迫方法和获得耐镉胁迫烟草植株方法。本发明提供了NtNRAMP2蛋白在创制耐镉胁迫烟草或选育耐镉烟草中的应用，NtNRAMP2蛋白的氨基酸序列如SEQ ID NO:2所示。本发明所述NtNRAMP2蛋白由NtNRAMP2基因编码合成，通过转基因及功能鉴定证实本发明所述的NtNRAMP2基因合成的NtNRAMP2天然抗性巨嗜细胞蛋白，与烟