Melanin precursor content determination method and application

The invention relates to a melanin precursor content determination method and application, the determination method is a high performance liquid chromatography, after a plant or edible fungus is extracted, sodium erythorbate and alcohol are added to prepare a test sample, the chromatographic condition is set as a C18 chromatographic column, and the column temperature is set as 30 DEG C; a mobile phase is a mixed solution containing methanol, acetonitrile and a 0.5% formic acid-water solution in a volume ratio of 30: 10: 60, the flow rate is 1mL/min, the detection wavelength is 300nm, the column temperature is 35 +/-1 DEG C, and the sample size is 5-7.5 mu L. The method has a good separation effect on melanin precursor substances 5, 6-dihydroxyindole carboxylic acid and 5, 6-dihydroxyindole in the plant or edible fungus extract, good repeatability and high precision, and can be used for preparing a novel method for separating melanin from the plant or edible fungus extract. And the universality is high. 本发明涉及一