Kit for visually and rapidly detecting four plasmodium based on RPA-CRISPR Cas12a system

The invention discloses a kit for visually and rapidly detecting four plasmodium based on an RPA-CRISPR Cas12a system, and belongs to the field of inspection and detection. The kit comprises an RPA universal primer pair, a crRNA fragment, a transverse flow immunochromatography reporter, a transverse flow chromatography test strip, a buffer solution for CRISPR reaction and Cas12a protein, the four kinds of plasmodium comprise plasmodium falciparum, plasmodium ovale, plasmodium vivax and plasmodium malaria. The invention also utilizes the kit to construct a method which is high in sensitivity, high in specificity, simple in operation method, rapid and accurate in detection of the four plasmodium. The method is suitable for field or clinical environments, especially in countries with limited resources, expensive instruments and equipment and professional standard laboratories are not needed, and the method has important significance on malaria monitoring, diagnosis, prevention and control. 本发明公开了基于RPA-CRISPR Cas