Thermal-stability Rnase inhibitor mutant, preparation method and application

The invention relates to the technical field of biology, in particular to a thermal stability Rnaseinhibator mutant, a preparation method and application, and the amino acid sequence of the Rnaseinhibator mutant is as shown in SEQ ID NO.1. The invention further discloses a preparation method of the...

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Bibliographische Detailangaben
Hauptverfasser: ZOU ZHENGZHENG, SONG DANFENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of biology, in particular to a thermal stability Rnaseinhibator mutant, a preparation method and application, and the amino acid sequence of the Rnaseinhibator mutant is as shown in SEQ ID NO.1. The invention further discloses a preparation method of the thermal stability Rnaseinhibator mutant. The activity is equivalent to that of wild type mouse source RI, but the thermal stability is obviously improved, and the heat resistance can reach 75 DEG C; and the residual activity exceeds 60% when the temperature is kept at 55 DEG C for 12 hours, so that a good condition is provided for the RNA high-temperature reaction. 本发明涉及生物技术领域,具体涉及一种热稳定性Rnaseinhibator突变体、制备方法及应用,Rnaseinhibator突变体的氨基酸序列如SEQ ID NO.1所示。活性与野生型鼠源RI相当,但热稳定性有显著提高,耐热能力可达到75℃;在55℃下保温12h,残余活力超过60%,为RNA高温反应提供了良好的条件。