Dual TB Green real-time fluorescent quantitative PCR (Polymerase Chain Reaction) universal primer group for identifying avian adenoviruses DAdV-3 and FAdV-4 and kit of dual TB Green real-time fluorescent quantitative PCR universal primer group

The invention relates to a dual TB Green real-time fluorescent quantitative PCR (Polymerase Chain Reaction) universal primer group for identifying fowl adenoviruses DAdV-3 and FAdV-4, which comprises the following components: DAdV-3-F: 5 '-ATCGAAGTGTCAGATGAAGGAGTG-3', DAdV-3-R: 5 '-CA...

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Bibliographische Detailangaben
Hauptverfasser:	CHENG LONGFEI, HUANG YU, LIN YU, CHEN ZHEN, LIU BINQIONG, SHI SHAOHUA, CAI GUOZHANG, FU GUANGHUA, CHEN CUITENG, ZHU CHUNHUA, WAN CHUNHE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a dual TB Green real-time fluorescent quantitative PCR (Polymerase Chain Reaction) universal primer group for identifying fowl adenoviruses DAdV-3 and FAdV-4, which comprises the following components: DAdV-3-F: 5 '-ATCGAAGTGTCAGATGAAGGAGTG-3', DAdV-3-R: 5 '-CACCATGGGGTTTAGTCGT-3', DAdV-3-R: 5 '- The invention relates to an avian adenovirus DAdV-3 and FAdV-4-R: 5 '-GGCTCCTCCTAC-3', FAdV-4-F: 5 '-GGCTCCTCCTAC-3', and FAdV-4-R: 5 '-AGATAGGCGGACACCAAAA-3'. The primer group can simultaneously identify clinically common DAdV-3 and FAdV-4, has good specificity and high sensitivity, can simplify the operation time and save the cost, and does not have the research report on the dual TB Green real-time fluorescent quantitative PCR identification of the avian adenovirus DAdV-3 and FAdV-4 at present, and can fill the blank in the field. 本发明涉及鉴别禽腺病毒DAdV-3和FAdV-4双重TB Green实时荧光定量PCR通用引物组，DAdV-3-F：5'-ATCGAAGTGTCAGATGAAGGAGTG-3'，DAdV-3-R：5'-CACCATCGGGGTTTAGTCGT-3'；FAdV-4-F：5'-GGCTCCTTGTTACCTCCCTCTAC-3