Human rhinovirus type B nucleic acid rapid detection system based on RT-RPA and CRISPR/Cas12a

The invention discloses a human rhinovirus type B nucleic acid rapid detection system based on RT-RPA (reverse transcription recombinase polymerase amplification) and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas12a (CRISPR/Cas12a). The detection system comprises an RT-RPA i...

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Hauptverfasser:	MO QIONG, JIAO SULI, LI YONGDONG, NI HONGXIA, XU GUOZHANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a human rhinovirus type B nucleic acid rapid detection system based on RT-RPA (reverse transcription recombinase polymerase amplification) and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas12a (CRISPR/Cas12a). The detection system comprises an RT-RPA isothermal amplification system and a CRISPR/Cas12a detection system, the RT-RPA isothermal amplification system comprises an RT-RPA primer pair designed on the basis of human rhinovirus B nucleic acid, and the RT-RPA primer pair comprises a forward primer and a reverse primer; the CRISPR/Cas12a detection system comprises crRNA (Ribonucleic Acid) which is designed on the basis of human rhinovirus type B nucleic acid. The CRISPR/Cas12a is adopted for detecting the nucleic acid sequence of the human rhinovirus B for the first time, and the method has the advantages of being high in sensitivity, high in specificity, short in consumed time, independent of large instruments and the like and is suitable for meeting the re