Primer and probe combination and method for detecting helicobacter pylori levofloxacin drug-resistant gene related mutation sites

The invention discloses a primer and probe combination and a method for detecting a mutation site related to a drug-resistant gene of helicobacter pylori levofloxacin. The primer and probe combination comprises primers as shown in SEQ ID No.1 to SEQ ID No.13 and probes as shown in SEQ ID No.14 to SEQ ID No.22. The invention further discloses a method for detecting the mutation site related to the drug-resistant gene of the helicobacter pylori levofloxacin. Wherein a locked nucleic acid base is added into the molecular beacon probe and is located at a position corresponding to mutation. According to the invention, a method for detecting the drug resistance of levofloxacin through combined use of mutation block amplification (ARMS), a Molecular Beacon and locked nucleic acid (LNA) is adopted, and through reasonable design and combination of primers and the probe, the detection purpose is achieved by adopting a landing fluorescence PCR technology. The method is high in sensitivity, strong in specificity, high in