MicroRNA detection method based on SplintR ligase triggered magnetic bead surface in-situ rolling circle amplification

The invention belongs to the technical field of biological detection, and particularly relates to a microRNA detection method based on magnetic bead surface in-situ rolling circle amplification triggered by SplintR ligase. Comprising the following steps: S1, oligonucleotide DNA single strands P1 and P2 form a P1-P2-miRNA double-strand compound with a target microRNA under the action of SplintR ligase, and then the double-strand compound is released into a P1-P2 single strand and the microRNA through heating denaturation; s2, adsorbing and enriching a P1-P2 single chain by using the streptavidin modified magnetic beads; s3, carrying out in-situ rolling circle amplification on the surface of the magnetic bead, wherein the P2 segment is used as an RCA reaction primer sequence in the in-situ rolling circle amplification; and S4, fluorescence detection. According to the method disclosed by the invention, two single-stranded DNAs are connected through a target microRNA bracket, and single-base mismatch recognition