Nanometer antibody specifically combined with pancreatic kininogenase and application thereof

The invention provides a nano antibody specifically bound with pancreatic kininogenase, and the nucleotide sequence of the nano antibody is shown as any one of SEQ ID No.1, SEQ ID No.3 and SEQ ID No.5. The invention further provides a corresponding application. The anti-kallidinogenase nano antibody with good expression activity is successfully screened and identified, and a pichia pastoris system is adopted for expression production, so that the production cost can be greatly reduced, and the industrialization of the antibody is facilitated; the purification filler taking the anti-kallidinogenase nano antibody as an accessory is successfully constructed, and a good foundation is laid for subsequent purification of kallidinogenase. 本发明提供了一种特异性结合胰激肽原酶的纳米抗体，其中，所述的纳米抗体的核苷酸序列如SEQ ID No.1、3、5任一所示。本发明还提供了相应的应用。本发明成功筛选鉴定出具有良好表达活性的抗胰激肽原酶纳米抗体，采用毕赤酵母系统进行表达生产，可大幅度地降低生产成本，更有利于该抗体的产业化；还成功构建了以抗胰激肽原酶纳米抗体作为配件的纯化填料，为后续纯化胰激肽原酶奠定良好的基础。