Preparation method of cord blood stem cells and prepared stem cell bank

The invention relates to the technical field of biology, in particular to a preparation method of cord blood stem cells and a prepared stem cell bank. The construction method of the stem cell bank comprises the following steps: 1) performing quality detection on cord blood stem cells; 2) treating th...

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Bibliographische Detailangaben
Hauptverfasser:	LIANG HAIYAN, DU SHAOYIN, JIA NAN, DING XIANFENG, LIU HAIXIA, LI JING, CHEN RUCHONG, LEE SI-YEOL, SHI XU, ZOU QINGYAN, ZOU YIJUN, ZUO WANLI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the technical field of biology, in particular to a preparation method of cord blood stem cells and a prepared stem cell bank. The construction method of the stem cell bank comprises the following steps: 1) performing quality detection on cord blood stem cells; 2) treating the qualified umbilical cord blood hematopoietic stem cells with a cryopreservation agent, and performing cryopreservation to obtain a human umbilical cord blood stem cell bank; the cryopreservation agent comprises a cell protective agent and a culture medium, wherein the cell protective agent comprises trehalose, albumin, glycerol and propylene glycol. The invention provides an umbilical cord blood stem cell transplantation scheme without blood type matching and HLA type matching for the first time. The standardized umbilical cord blood stem cell bank is mainly used for improving the success rate of stem cell transplantation and guiding establishment of stem cell treatment schemes of related diseases or research of