Efficient fraxinus mandshurica globular embryo induction and proliferation method

The invention discloses an efficient fraxinus mandshurica globular embryo induction and proliferation method, which is characterized in that fraxinus mandshurica single-cell or small-cell mass is selected as a material, and the fraxinus mandshurica globular embryo is induced and proliferated by a two-step method, and comprises the following steps: firstly, inoculating the single-cell or small-cell mass into a WPM culture medium containing 2, 4-D and potassium citrate, and carrying out shake culture for 7-8 weeks under a dark condition; and then inoculating into a WPM culture medium containing potassium citrate, and carrying out shake culture under a dark condition, so that the culture continues to form globular embryos and proliferate. The establishment of the method solves the limitation of material sources in fraxinus mandshurica somatic embryogenesis induction, lays a foundation for large-scale propagation of fraxinus mandshurica somatic embryogenesis ways, and has important significance for vegetative pro