Preparation and application of nucleic acid extraction material based on CTAB (Cetyltrimethyl Ammonium Bromide) modified attapulgite
The invention discloses preparation and application of a nucleic acid extraction material based on CTAB (Cetyltrimethyl Ammonium Bromide) modified attapulgite, and belongs to the technical field of biology. The method comprises the following specific steps: S1, acid activation modification: stirring...
Gespeichert in:
Hauptverfasser: | , , , , , , , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention discloses preparation and application of a nucleic acid extraction material based on CTAB (Cetyltrimethyl Ammonium Bromide) modified attapulgite, and belongs to the technical field of biology. The method comprises the following specific steps: S1, acid activation modification: stirring attapulgite powder and an HCl aqueous solution to react, filtering, washing a solid, drying, and then grinding and sieving to obtain acid activated attapulgite; and S2, CTAB modification: adding CTAB and the acid activated attapulgite into hydrochloric acid, stirring, adjusting the pH value to be neutral, drying the solution, grinding and drying to obtain a solid, and sieving to obtain the CTAB modified attapulgite. According to the method, the attapulgite is modified by CTAB, the physicochemical properties of the attapulgite are optimized, the nucleic acid adsorption performance of the attapulgite is greatly improved, and meanwhile, the low cost is ensured.
本发明公开了一种基于CTAB改性凹凸棒土的核酸提取材料的制备及应用,属于生物技术领域。该方法具体步骤如下:S1、 |
---|