Method for producing cell mass

A method for producing a cell mass, in which adherent culture is performed on a cell culture substrate having two regions (A) and (B), said method having steps (1)-(3). The present invention relates to a method for producing mesenchymal cells, (A) an island-like region having a cell-proliferative property and an area of 0.001-1 mm2, (B) a region adjacent to the region (A) and not having a cell-proliferative property, (1) a step for preparing a cell suspension containing mesenchymal cells and ectoderm-derived cells, (2) a step for bringing the cell suspension into contact with a cell culture substrate to cause the two types of cells to randomly adhere to the region (A), and (3) a step for culturing the cells in the region (A). The total number of the two types of cells on the contact surface of the cell suspension and the cell culture substrate is 100-100,000 cells/cm2, and (3) a step for culturing the cells adhered to the region (A) to form a cell mass containing the two types of cells. 一种细胞块的制造方法，其在细胞培养基材上进行