Novel feed and test method for enhancing immunity of Roman laying hens under heat stress
The invention belongs to the technical field of novel feeds for poultry breeding, and discloses a novel feed and a test method for enhancing the immunity of Roman laying hens under heat stress, the method comprises the following steps: collecting a wing vein blood sample of the Roman laying hens, ce...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention belongs to the technical field of novel feeds for poultry breeding, and discloses a novel feed and a test method for enhancing the immunity of Roman laying hens under heat stress, the method comprises the following steps: collecting a wing vein blood sample of the Roman laying hens, centrifuging to obtain serum, and detecting the concentration of corticosterone serum by using an ELISA (Enzyme-Linked Immunosorbent Assay) kit; preparing a blood smear, dyeing, and respectively counting white blood cells and lymphocytes; the ELISA kit is adopted to detect the IgG and IgM levels of chicken serum immune globulins, and inflammatory cytokines, antioxidants and spleen functions are respectively evaluated. By analyzing the special influence of the curcumin added into the feed on the immune response of the hens under the heat stress condition, it is determined that the special influence of the curcumin added into the feed on the immune response of the hens under the heat stress condition is reduced, and th |
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