Separation method and application of N-Boc-L-ethyl pyroglutamate enantiomer

The invention discloses a separation method and application of an N-Boc-L-ethyl pyroglutamate enantiomer, and the separation method comprises the following steps: a test sample containing the N-Boc-L-ethyl pyroglutamate enantiomer is taken, and is separated through liquid chromatography, and the liquid chromatography conditions are as follows: a Daxitrin chromatographic column AD-H is used as a stationary phase, and the column temperature is 30-50 DEG C; a mixed solution containing n-hexane and absolute ethyl alcohol is used as a mobile phase for isocratic elution, and the volume ratio of n-hexane to absolute ethyl alcohol in the mobile phase is (3-9): 1. Effective separation of the N-Boc-L-ethyl pyroglutamate and the enantiomer thereof can be realized through chromatographic conditions of the scheme, and the separation degree of the N-Boc-L-ethyl pyroglutamate and the enantiomer thereof can reach 9.77 or above. Meanwhile, the retention time of the N-Boc-L-ethyl pyroglutamate in the scheme is short, rapid and