Establishment of traditional Chinese medicine compound UPLC fingerprint spectrum, fingerprint spectrum and application

The method comprises the following steps: preparing a test solution from a water-soluble part of the licorice heart-fire purging decoction, mixing reference substances of licorice, scutellaria baicalensis, dried ginger, jujube, pinellia ternate and coptis chinensis to form a reference substance solution, taking a formic acid aqueous solution with the volume fraction of 0.4% as a mobile phase A and acetonitrile as a mobile phase B, carrying out UPLC chromatographic condition sample introduction to obtain a chromatogram, and carrying out system analysis to obtain the UPLC fingerprint spectrum. According to the invention, water-soluble parts are purified from the whole formula of the licorice heart-fire purging decoction, and a corresponding UPC fingerprint spectrum is established, and the result shows that the UPLC fingerprint spectrum provided by the invention has excellent reproducibility and accuracy, has 46 common peaks, can comprehensively detect the drug effect, can accurately and efficiently detect the d