Primer combination, kit and method for detecting tize pathogen based on LAMP-LFD (loop-mediated isothermal amplification-lateral flow dipstick)

The invention provides a primer combination, a kit and a method for detecting tize pathogen based on LAMP-LFD (loop-mediated isothermal amplification-lateral flow dipstick), and belongs to the technical field of medical molecular biological detection. The primer combination provided by the invention comprises a forward outer primer F3, a reverse outer primer B3, a forward inner primer FIP, a reverse inner primer BIP, a loop primer LF and a probe PB1. The invention also provides a kit and a method for detecting the tize pathogen based on the primer combination, the detection of the tize pathogen can be realized, and the detection method is simple, short in time consumption, convenient in result judgment and high in detection accuracy and sensitivity. 本发明提供了一种基于LAMP-LFD检测泰泽病原体的引物组合、试剂盒及方法，属于医学分子生物学检测技术领域。本发明提供的引物组合包括正向外引物F3、反向外引物B3、正向内引物FIP、反向内引物BIP、环引物LF和探针PB1。本发明还提供了基于上述引物组合的检测泰泽病原体的试剂盒和方法，可实现对泰泽病原体的检测，并且检测方法简单，耗时短，结果便于判断，检测准确性和灵敏度高。