Femoral head internal partitioning method for comparing normal and necrotic femoral head internal space structures and bone metabolism

The invention belongs to the field of medical experiments, and provides a femoral head internal partitioning method for comparing normal and necrotic femoral head internal space structures and bone metabolism, which comprises the following steps: collecting a femoral head specimen and partitioning;...

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Bibliographische Detailangaben
Hauptverfasser: SUN LEI, YANG PEICHUAN, MA JIANXIONG, MA XINLONG, LI YAN, WANG YAN, DONG BENCHAO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of medical experiments, and provides a femoral head internal partitioning method for comparing normal and necrotic femoral head internal space structures and bone metabolism, which comprises the following steps: collecting a femoral head specimen and partitioning; scanning by adopting Micro-CT (Computed Tomography) and quantitatively calculating; paraffin sectioning and HE dyeing; performing immunohistochemical staining; clinical specimens of the normal femoral head and the necrotic femoral head are collected and cut into four corresponding areas, Micro-CT scanning and quantitative calculation, paraffin section HE staining and OPG and BMP2 immunohistochemical staining are used for observing and analyzing bone trabecular space structures and metabolic activity conditions of different areas corresponding to the normal femoral head and the necrotic femoral head, and the clinical specimens of the normal femoral head and the necrotic femoral head are obtained. Starting from clini