Kit for detecting number abnormality of autosomes and sex chromosomes

The invention relates to the field of molecular biology diagnosis, in particular to a kit for detecting number abnormality of autosomes and sex chromosomes and application of the kit. The kit comprises a primer pair as shown in SEQ ID (Sequence Identity). NO.1-236. The kit comprises a primer pair as...

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Hauptverfasser: SHI RUYUN, QIU YIFAN, CHEN YUWEI, XIAO YUQIAO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the field of molecular biology diagnosis, in particular to a kit for detecting number abnormality of autosomes and sex chromosomes and application of the kit. The kit comprises a primer pair as shown in SEQ ID (Sequence Identity). NO.1-236. The kit comprises a primer pair as shown in SEQ ID. According to the invention, a plurality of short tandem repeat sequences on chromosomes and chromosome specific detection sites are amplified at the same time in one PCR reaction, PCR products are analyzed through a high-resolution technology, such as capillary electrophoresis, and whether the number of chromosomes is normal or abnormal is judged by calculating the area of a detection peak. Starting from a DNA sample, a result can be obtained within 3 hours, the number of all autosomes and sex chromosomes can be detected at the same time only through one PCR experiment, the detection technology flow is simple, and standardization is easy to achieve. 本发明涉及分子生物学诊断领域,具体涉及一种用于检测常染色体和性染色体数目异常的试剂盒及其应用。所