Construction method of recombinant porcine epidemic diarrhea virus infectious clone as well as infectious clone and application thereof

The invention discloses a construction method of a recombinant porcine epidemic diarrhea virus infectious clone as well as the infectious clone and application thereof. The construction method comprises the following steps: carrying out first-round gene recombination to amplify a targeting fragment; electrically transforming the targeting fragment to obtain a positive clone; the obtained positive clone is subjected to second-round recombination to remove enzyme cutting sites and resistance genes, and the PEDV infectious clone expressing the target gene is obtained. A PEDV reverse genetics system based on bacterial artificial chromosomes is established, PEDV genomes are rapidly and efficiently edited in vitro through a Red homologous recombination technology, a powerful technical platform is provided for researching molecular biological characteristics and pathogenesis of viruses and developing genetic engineering vaccines, and the method is efficient, convenient, high in specificity and good in application pr