Construction of KLHL34 knockout cell line and application of KLHL34 knockout cell line as small RNA viridae virus production cell line

The invention belongs to the field of gene engineering, and particularly relates to construction of a KLHL34 knockout cell line and application of the KLHL34 knockout cell line as a small RNA viridae virus vaccine production cell line. Firstly, it is found that replication of small RNA viridae virus FMDV can be promoted by inhibiting KLHL34 gene expression in host cells; secondly, the invention provides sgRNA specifically targeting KLHL34, the sgRNA can specifically target the KLHL34 gene, the knockout of the KLHL34 gene is realized by combining a CRISPR-Cas9 technology, and the obtained monoclonal cell line can significantly promote the replication of the small RNA viridae virus FMDV, improve the production quantity and antigen yield of the FMDV vaccine, and can be used for the preparation of the FMDV vaccine. The cell line can be used as a production cell line of picornaviridae virus vaccines, especially FMDV vaccines, and has a wide application prospect. 本发明属于基因工程领域，具体涉及KLHL34敲除细胞系的构建及作为小RNA病毒科病毒疫苗生产细胞系的应用