Gene modified CAR-NK cell as well as preparation method and application thereof

The invention provides a gene modified CAR-NK cell as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering. A recombinant expression vector containing CAR-Lewis-Y is adopted to infect a general type NK cell to obtain a CAR-NK cell; the universal NK cell is an NK cell in which a TIGIT gene and an ADORA2A gene are knocked out and IL-18 is expressed; the invention also provides a preparation method and application of the CAR-NK cell. After the CAR-Lewis-Y-NK cell prepared by the invention and a target cell HCC-827 are subjected to mixed culture for 72 hours, the secretion amount of cell factors TNF-alpha and IFN-gamma is obviously increased. The killing rate of the CAR-Lewis-Y-NK cell prepared by the invention to HCC-827 cells is 91.3%, and the killing rate of the CAR-Lewis-Y-NK cell to LOVO cells is 92.1%. 本发明提供一种基因修饰的CAR-NK细胞及其制备方法和应用，属于遗传工程技术领域；采用含CAR-Lewis-Y的重组表达载体感染通用型NK细胞得到CAR-NK细胞；所述通用型NK细胞为敲除TIGIT基因和敲除ADORA2A基因，并表达IL-18的NK细胞；本发明还提供上述CAR-NK细胞的制备方法