Design method of novel probe CazFPC-SNAP and visual detection of protein aggregate by using novel probe CazFPC-SNAP

The invention discloses a design method of a novel probe CazFPC-SNAP, which is used for visual detection of protein aggregate. The CazFPC-SNAP is synthesized by a three-step method of 4-hydroxybenzylideneimidazolinone parent nucleus conjugated phenylboronic acid pinacol ester and anchoring a biological label SNAP. Wherein pinacol phenylboronic acid ester is used as a hydrogen peroxide recognition site, and the introduced biotag SNAP is used for recognizing and marking a target protein. The probe CazFPC-SNAP not only can be used for rapidly detecting the concentration level of hydrogen peroxide, but also can realize visual imaging of protein misfolding aggregation in living cells, has obvious fluorescence response to viscosity change in a protein folding system, and can identify protein aggregates with naked eyes; the fluorescent probe has relatively low cytotoxicity, good biocompatibility and stable fluorescent property; the method has very important application in the fields of biological imaging, fluorescen