Ultrasonic stimulation method for improving preservation effect of in-vitro osteochondral tissue

The invention discloses an ultrasonic stimulation method for improving an in-vitro osteochondral tissue preservation effect, and belongs to the technical field of cartilage tissue preservation. The method comprises the following steps that articular cartilage tissue with subchondral bones of an animal or a human is obtained in vitro and placed in a sterile culture bag containing a tissue culture solution, ultrasonic stimulation is applied to the articular cartilage tissue, and the ultrasonic stimulation method comprises the steps that according to the combined parameters of the stimulation intensity being 0.1-3000 mW/cm < 2 >, the frequency being 0.1-5 MHz, the duty ratio being 1%-60% and the stimulation time being 1-60 min/time, ultrasonic stimulation is conducted on the articular cartilage tissue, the osteochondral tissue is subjected to 2-5 times of ultrasonic stimulation every week in combination with environmental factors such as temperature and air pressure. According to the method provided by the inven