Aptamers against SARS-CoV-2

The present invention relates to one or more isolated aptamers directed against the SARS-CoV-2 spike protein and methods of use thereof. Certain embodiments of the invention relate to methods for detecting the presence, absence, or content of SARS-CoV-2 in a sample using one or more aptamers described herein. In certain embodiments, the present invention relates to one or more aptamers capable of specifically binding to the SARS-CoV-2 protein, including aptamers capable of specifically binding to the S1 subunit (including the receptor binding domain (RBD)) and/or the S2 subunit, the S1 subunit and/or the S2 subunit as part of the SARS-CoV-2 spike protein in a trimeric form in their native conformation, or as separate monomers. 本发明涉及针对SARS-CoV-2刺突蛋白的一种或多种分离的适体及其使用方法。本发明的某些实施例涉及使用本文所述的一种或多种适体检测样本中SARS-CoV-2的存在、不存在或含量的方法。在某些实施例中，本发明涉及一种或多种能够特异性结合SARS-CoV-2蛋白的适体，包括能够特异性结合S1亚基(包括受体结合域(RBD))和/或S2亚基的适体，该S1亚基和/或S2亚基以其天然构象作为三聚体形式的SARS-CoV-2刺突蛋白的一部分，或作为单独的单体。