Method for rapidly extracting fat in high oleic acid peanut and determining fatty acid composition
The invention relates to the technical field of fatty acid determination, and discloses a method for rapidly extracting fat in high-oleic-acid peanuts and determining fatty acid composition, high-oleic-acid peanut kernels are selected, dried and crushed, folded and wrapped with filter paper and put...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to the technical field of fatty acid determination, and discloses a method for rapidly extracting fat in high-oleic-acid peanuts and determining fatty acid composition, high-oleic-acid peanut kernels are selected, dried and crushed, folded and wrapped with filter paper and put into a brown bottle, petroleum ether is added, ultrasonic-assisted extraction is performed under the conditions of set power, temperature and time, and the content of fatty acid in the peanut kernels is determined. After extraction, putting a sample into a Soxhlet extractor, turning on condensed water flow, turning on a petroleum ether reflux switch, after extraction is completed, taking out the filter paper bag, heating an extraction bottle to remove petroleum ether so as to obtain peanut fat, adding a petroleum ether-diethyl ether mixed solution, standing, then adding sodium hydroxide-methanol, fully shaking up, standing, taking supernate, and drying, thereby obtaining the peanut fat. And putting into a gas chrom |
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