Method for determining influence of dsRNA on direct toxicity of spider mites

The invention discloses a method for determining the influence of dsRNA on the direct toxicity of spider mites, and belongs to the technical field of agricultural biology. Comprising the following steps: mixing dsRNA aiming at related target genes with a Tween solution, soaking tetranychid mite eggs so as to introduce the dsRNA into tetranychid mite bodies, and determining the toxicity of the exogenous dsRNA to the tetranychid mites; the method is used for researching gene functions and evaluating the toxicity of exogenous dsRNA to tetranychid mites; the method can effectively determine the direct toxicity influence of the exogenous dsRNA on the spider mites, is simple and feasible, has good effectiveness and sensitivity, and has important significance and application prospects for research of related gene functions and RNAi target gene screening. 本发明公开了一种dsRNA对叶螨直接毒性影响的测定方法，属于农业生物技术领域；包括将针对相关靶基因的dsRNA与吐温溶液混合后浸泡叶螨卵，以使dsRNA导入到叶螨体内，并测定外源dsRNA对叶螨的毒性；该方法用以研究基因功能和评价外源dsRNA对叶螨的毒性；该方法能够有效测定外源dsRNA对叶螨的直接毒性影响，且方法简单可行，