Hematoxylin staining solution as well as preparation method and application thereof

The invention discloses hematoxylin staining fluid as well as a preparation method and application thereof. The hematoxylin staining solution is prepared from the following raw materials in percentage by mass: 0.4 percent to 0.6 percent of hematoxylin, 0.02 percent to 0.04 percent of citric acid, 25...

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Bibliographische Detailangaben
Hauptverfasser: HU YUANYUAN, KIM HWANG-KEUN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses hematoxylin staining fluid as well as a preparation method and application thereof. The hematoxylin staining solution is prepared from the following raw materials in percentage by mass: 0.4 percent to 0.6 percent of hematoxylin, 0.02 percent to 0.04 percent of citric acid, 25 percent to 30 percent of diglycerol, 1.4 percent to 1.8 percent of acetic acid, 0.02 percent to 0.04 percent of aluminum oxide, 0.5 percent to 0.7 percent of sodium iodate, 16 percent to 18 percent of absolute ethyl alcohol, 0.8 percent to 1.2 percent of inert silicon dioxide, 2.8 percent to 3.5 percent of aluminum sulfate and 44.12 percent to 53.06 percent of distilled water. The preparation method comprises the following steps: dissolving hematoxylin and absolute ethyl alcohol to obtain a solution 1; dissolving diglycerol, aluminum sulfate and half of distilled water to obtain a solution 2; dissolving inert silicon dioxide, sodium iodate and the other half of distilled water to obtain a solution 3, and adding si