Flanking sequence of rice GAT conversion event GATV3-34-3 as well as detection primer and application thereof
The invention relates to a flanking sequence of a rice GAT conversion event GATV3-34-3 as well as a detection primer and application of the flanking sequence. The flanking sequence provided by the invention comprises a right flanking sequence as shown in SEQ ID NO. 1 and a left flanking sequence as...
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| creator | SUN TONG ZHAO HUIMIN ZHAO GUANGMIAO ZENG XIANG HUANG PEIJING OUYANG CHAO JIN XIONGXIA LI XINPENG |
| description | The invention relates to a flanking sequence of a rice GAT conversion event GATV3-34-3 as well as a detection primer and application of the flanking sequence. The flanking sequence provided by the invention comprises a right flanking sequence as shown in SEQ ID NO. 1 and a left flanking sequence as shown in SEQ ID NO. 2. The invention provides specific primers SEQ ID NO. 9 to 10 or SEQ ID NO. 11 to 12 for detecting the flanking sequences. The specific primer provided by the invention is used for carrying out PCR (Polymerase Chain Reaction) amplification on a DNA (Deoxyribose Nucleic Acid) sample of rice to be detected, so that whether a T-DNA fragment of pC0309-KhvMaauMCMK5400 is inserted into the 32791293 to 32791426 basic groups of a non-coding region of the No.4 chromosome of the rice or not can be specifically indicated. According to the invention, detection and safety management of the transgenic rice and the derivative line thereof are successfully realized.
本发明涉及一种水稻GAT转化事件GATV3-34-3的侧翼序列及其检测引物与应用。本发明提 |
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本发明涉及一种水稻GAT转化事件GATV3-34-3的侧翼序列及其检测引物与应用。本发明提</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Flanking sequence of rice GAT conversion event GATV3-34-3 as well as detection primer and application thereof |
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