Acquisition method and application of chicken primordial germ cells for breed conservation of strain resources

The invention discloses an acquisition method and application of chicken primordial germ cells for strain resource breed conservation. An EAV-HP gene is inserted into the SLCO1B3 gene locus of the cell, the insertion sequence is shown as SEQ ID NO: 1, and the cell has a transient expression green fl...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG ZHE, WU ZHAOTAO, TANG FEITAI, TANG XIAOCHUAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an acquisition method and application of chicken primordial germ cells for strain resource breed conservation. An EAV-HP gene is inserted into the SLCO1B3 gene locus of the cell, the insertion sequence is shown as SEQ ID NO: 1, and the cell has a transient expression green fluorescent protein marker. The cell is obtained by the following method: separating gonads from embryos of a green-shell laying hen strain needing breed conservation, digesting by pancreatin, separating and purifying primordial germ cells by a differential adhesion method, and transferring into a green fluorescent protein gene through an adenovirus vector. The cell can be preserved in liquid nitrogen for a long time at low cost. The cells are microinjected into a Bailaiaviation chick embryo, a chimeric chicken can be obtained after hatching, and a green-shell laying hen strain living body needing breed conservation can be obtained again from offspring of the chimeric chicken. The method has important application val