Method for in vitro amplification of adherent cells

The invention relates to the technical field of cell culture, in particular to an adherent cell in-vitro amplification method. After cleaning and sterilizing the paper carrier and the bioreactor, adding an adherent cell culture medium for culturing; in the culture process, consumption of nutrient substances required by adherent cell culture is maintained in a mode of changing a culture solution by adding supplementary materials into the culture solution every day or observing the color of the culture solution, and meanwhile, the bottom propeller provides thrust to the paper carrier, so that the paper carrier is maintained in a suspended state, and the adherent cell culture is realized. The carrier bracket does not make contact with devices except for the carrier bracket; after culture is finished, the exosome is sucked out through a liquid suction pipe; and after culture is finished, pancreatin is added into the tank to digest and disperse the adherent cells, the adherent cells are sucked out from the liquid