Anticoagulant protein Qinghaienin of ticks and application of coding gene of anticoagulant protein Qinghaienin in anticoagulation

Anticoagulant protein Qinghaienin of ticks and application of coding gene of anticoagulant protein Qinghaienin in anticoagulation

The invention relates to the technical field of biology, and provides an anticoagulant protein Qinghaienin of ticks and an application of a coding gene of the anticoagulant protein Qinghaienin in anticoagulation, and the anticoagulant protein is an anticoagulant protein Qinghaienin of haemaphysalis...

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Hauptverfasser: GAO JINLIANG, CAO MEINA, BAI MENGDIE, YANG YINRAN, CHEN KAITING, GAO DONGBING, SU MUYA, SHE ZHANFEI, ZHAO WENBIN, HE XIAOFEI, HIROKEI, MA LINYUAN
Format: Patent
Sprache:chi ; eng
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BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention relates to the technical field of biology, and provides an anticoagulant protein Qinghaienin of ticks and an application of a coding gene of the anticoagulant protein Qinghaienin in anticoagulation, and the anticoagulant protein is an anticoagulant protein Qinghaienin of haemaphysalis qinghaiensis and has an amino acid sequence as shown in SEQ ID NO.1. The invention further provides a preparation method of the anticoagulant protein Qinghaienin of the ticks and an application of the coding gene of the anticoagulant protein Qinghaienin of the ticks. The invention also provides a coding gene Hq021 of the haemaphysalis qinghaiensis anticoagulant protein Qinghaienin. The Hq021 gene comprises a nucleotide sequence SEQ ID NO.2 for coding the amino acid sequence as shown in SEQ ID NO.1. The invention also provides a preparation method of the haemaphysalis qinghaiensis anticoagulant protein Qinghaienin. The gene is expressed in escherichia coli, and nickel ion affinity chromatography resin is used for pu