High-throughput detection method for SAM content and application of high-throughput detection method

The invention discloses a high-throughput detection method of SAM content and application thereof, and belongs to the technical field of biology. The method comprises the following steps: adding a premixed solution into a to-be-detected solution containing SAM for reaction, then adding a glycine oxidase detection solution for reaction after the reaction is finished, and detecting the generation amount of hydrogen peroxide in a reaction product to obtain the SAM content, through combination of glycine/sarcosine N-methyltransferase and glycine oxidase, the SAM content in the range of 0-2mM can be accurately detected on a 96 microwell plate, and the method is accurate in detection, proper in color development range and small in influence of other factors. The method is successfully applied to high-throughput screening of the SAM synthetase, a plurality of mutants with higher SAM accumulation are screened by taking the wild type SAM synthetase as a template, and the method can be used for industrial production of