Method for extracting protein from excess sludge through hydrolysis catalysis of thermophilic bacteria

The method comprises the following steps: (1) selecting thermophilic bacteria DC8, inoculating the thermophilic bacteria DC8 into a solid culture medium, culturing the thermophilic bacteria DC8 at 60 DEG C for 24 hours, inoculating the thermophilic bacteria DC8 into 250 mL of a liquid culture medium...

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Hauptverfasser: ZHONG WEIZHANG, NING ZHIFANG, DONG XILEI, WANG CHANGWEI, DING YATING, LI YANFEI, ZHANG HAORAN, HOU JIWEI, WANG CHAOYANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The method comprises the following steps: (1) selecting thermophilic bacteria DC8, inoculating the thermophilic bacteria DC8 into a solid culture medium, culturing the thermophilic bacteria DC8 at 60 DEG C for 24 hours, inoculating the thermophilic bacteria DC8 into 250 mL of a liquid culture medium, culturing the thermophilic bacteria DC8 at 60 DEG C for 24 hours, and enabling the absorbance of the thermophilic bacteria DC8 at the wavelength of 600 nm to reach 1.0 to obtain a thermophilic bacteria suspension; (2) centrifuging the thermophilic bacterium suspension to obtain a thermophilic bacterium crude enzyme; (3) adjusting the pH value of the residual sludge to 7, mixing the residual sludge with the thermophilic bacteria crude enzyme solution in a reactor, oscillating at 60 DEG C and 120 r/min, and reacting for 6-12 hours; the solid content of the excess sludge is 2%-5%, and the addition amount of the thermophilic bacteria crude enzyme liquid is 5%-25%; and (4) after the reaction is finished, centrifuging