Method for improving content of resveratrol in vitis davidii cells through gene editing and constructed vitis davidii CHS2 mutant cell line

The invention belongs to the field of plant genetic engineering, and particularly relates to a method for improving the content of resveratrol in vitis davidii cells through gene editing and a constructed vitis davidii CHS2 mutant cell line, and the vitis davidii chalcone synthase gene CHS2 is subje...

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Hauptverfasser: PINGEOT, LI SIYU, LAI CHENGCHUN, HE LIYUAN, QUE QIUXIA, LAI GONGTI, XU HENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of plant genetic engineering, and particularly relates to a method for improving the content of resveratrol in vitis davidii cells through gene editing and a constructed vitis davidii CHS2 mutant cell line, and the vitis davidii chalcone synthase gene CHS2 is subjected to gene editing by adopting a CRISPR/Cas9 method to construct the vitis davidii CHS2 mutant cell line. According to the invention, expression of key genes in a target metabolic pathway and accumulation of metabolites can be reduced, biosynthesis of resveratrol in a competitive metabolic pathway is indirectly promoted, and the content of resveratrol is increased by 2-4 times. According to the invention, the problems of high cost, limited plant resources, long growth cycle, seasonal variation, uncontrollable environmental factors and the like confronted by synthesis of natural plant resveratrol are solved; meanwhile, the problems of impurity pollution, toxic substance residue, high edible and medicinal safety ri