Antibody specifically aiming at isohydroxy digitonin and related application thereof

The invention relates to a novel monoclonal antibody, a preparation method of the antibody and related application of the antibody in immunodetection. The monoclonal antibody disclosed by the invention can aim at isohydroxy digitonin (DIG) or isohydroxy digitonin (digoxin) with high sensitivity and high specificity, so that the monoclonal antibody not only can be used for detecting target molecules such as nucleic acid molecules marked by DIG, but also can be used for detecting medicine molecules digoxin; therefore, the method can be beneficial to judging digoxin poisoning and guiding clinical medication, and has important significance for ensuring the treatment effectiveness and safety of digoxin. 本发明涉及一种新的单克隆抗体、该抗体的制备方法、及其用于免疫检测的相关应用。本发明的单克隆抗体能够高灵敏度且高特异性地针对异羟基洋地黄毒甙元(DIG)或异羟基洋地黄苷(地高辛)，使其不仅能够用于经DIG标记的目标分子如核酸分子的检测，也能够用于药物分子地高辛的检测，并由此能够有助于判断地高辛中毒及指导临床用药，对保证地高辛的治疗有效性及安全性具有重要意义。