Devices, methods and kits for microfluidic assays

Methods, systems, and kits for determining dissolved oxygen levels within a microfluidic device are provided. Microfluidic devices may be suitable for cell culture. The methods, systems, and kits may also be used to determine oxygen consumption levels in a population of biological micro-objects. In particular, the methods, systems and kits of the present disclosure depend on flowing a fluid medium comprising a dye and a supplied partial pressure of oxygen into a microfluidic device for a period of time wherein fluorescence emitted by the dye varies based on the availability of oxygen near the dye; acquiring a fluorescence image of an indoor region of interest; and correlating the fluorescence of the fluorescence image of the region of interest with a reference to determine the oxygen partial pressure observed in the region of interest. 提供了用于确定微流体装置内的溶解氧水平的方法、系统和试剂盒。微流体装置可以适用于细胞培养。该方法、系统和试剂盒还可以用于确定生物微物体群中的耗氧水平。特别地，本公开的方法、系统和试剂盒依赖于使包含染料和供应的氧分压的流体介质流入微流体装置中持续一时间段，其中，由染料发射的荧光基于染料附近的氧的能用性而改变；获取室内的受关注区域的荧光图像；以及将受关注