Extraction, identification, development and application of nano antibody in alpaca and camel blood

The invention relates to the technical field of nano antibodies, in particular to extraction, identification, development and application of nano antibodies in alpaca and camel blood, which comprises the following steps: protein extraction: extracting proteins from blood samples by using a UA lysis method, and quantifying the proteins by using BCA; the method comprises the following steps of: performing enzymolysis in a protein solution, desalting a peptide fragment by adopting MCX after the protein in a blood sample is subjected to enzymolysis, adding 40 microliters of 0.1% formic acid solution for redissolving after the peptide fragment is freeze-dried, quantifying the peptide fragment, loading on a mass spectrum, separating on the mass spectrum by adopting a nanoliter flow rate HPLC (High Performance Liquid Chromatography) liquid phase system Easy nLC, loading the sample to a chromatographic column Thermo-specific EASY colomn, and then separating through an analytical column. Nanometer antibodies are ident