Method for detecting 4-bromobutyric acid in ethyl 4-bromobutyrate

The invention provides a method for detecting 4-bromobutyric acid in ethyl 4-bromobutyrate, and relates to the field of pharmaceutical analysis. The method comprises the following steps: carrying out isocratic elution at a flow rate of 0.9-1.1 ml/min by adopting an amino bonded silica gel chromatographic column; the mobile phase is 10-40mM ammonium formate solution or ammonium acetate solution, and the ratio of the ammonium formate solution or ammonium acetate solution to acetonitrile is 5: 95. The detection method for the 4-bromobutyric acid in the ethyl 4-bromobutyrate can effectively detect the content of the 4-bromobutyric acid in the ethyl 4-bromobutyrate, is good in specificity, high in sensitivity and high in durability, makes up the blank of the prior art, and has important significance on quality control of the ethyl 4-bromobutyrate and clinical medication safety of the oxaggolil sodium. 本发明提供一种4-溴丁酸乙酯中4-溴丁酸的检测方法，涉及药物分析领域。该方法包括采用氨基键合硅胶色谱柱，以0.9～1.1ml/min的流速进行等度洗脱；流动相为10～40mM甲酸铵溶液或乙酸铵溶液：乙腈＝5:95。本发明提供的4