Pretreatment method and application of bone marrow tissue

The invention provides a pretreatment method and application of bone marrow tissue. According to the pretreatment method, the marrow tissue is soaked in EDTA decalcification liquid to achieve first-time complete exposed decalcification, second-time marrow tissue surface decalcification is conducted through hydrochloric acid decalcification liquid, the good decalcification effect can be achieved, the section is not affected, meanwhile, the antigen is not damaged, the pretreated marrow tissue can be subjected to FISH detection, and the detection accuracy is high. And false negative can be effectively avoided. 本发明提出了一种骨髓组织的预处理方法及应用；所述预处理方法通过浸泡在EDTA脱钙液实现第一次骨髓组织完全裸露脱钙，通过盐酸脱钙液进行第二次骨髓组织表面脱钙，既能够实现好的的脱钙效果，又能够不影响切片的同时不破坏抗原，预处理后的骨髓组织可以进行FISH检测，可有效避免假阴性。