Plasmid vector and preparation method of MS2 phage-like particle for displaying foreign protein

The invention relates to the technical field of biological medicine, in particular to a plasmid vector and a preparation method of an MS2 phage similar particle for displaying foreign protein. The plasmid vector provided by the invention is characterized by sequentially containing an arabinose promoter, an MS2CP gene, an amber codon, a Linker sequence, a foreign protein gene insertion part, a His tag coding sequence, an MS2 bacteriophage packaging sequence, a hammerhead enzyme sequence and a mature protein sequence for coding the MS2 bacteriophage. The plasmid vector can be used for preparing MS2 bacteriophage-like particles of which the surfaces display foreign proteins, and can be applied to preparation of MS2 bacteriophage subunit vaccines. 本发明涉及生物医药技术领域，具体为一种质粒载体及展示外源蛋白的MS2噬菌体类似颗粒的制备方法。本发明提供的质粒载体的特征在于：该质粒载体依次含有阿拉伯糖启动子，MS2CP基因、琥珀密码子、Linker序列、外源蛋白基因插入部分、His标签编码序列、MS2噬菌体包装序列、锤头酶序列和编码MS2噬菌体成熟蛋白序列。通过该质粒载体可以制备表面展示外源蛋白的MS2噬菌体类似颗粒，可应用于MS2噬菌体亚单位疫苗的制备。