Method for expressing D-amino acid oxidase and application of D-amino acid oxidase
The invention relates to the technical field of biochemical engineering, in particular to a method for expressing D-amino acid oxidase and application of the D-amino acid oxidase. Wild type D-amino acid oxidase with catalytic activity on a catalytic substrate DL-glufosinate-ammonium is obtained thro...
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| creator | XU JIAQI LOU YIYUAN WANG ZIYUAN ZHOU SHUGUANG YANG LIRONG YU SHENLUAN ZHANG ZEWEI JIN YONG ZHOU HAISHENG KANG XIN |
| description | The invention relates to the technical field of biochemical engineering, in particular to a method for expressing D-amino acid oxidase and application of the D-amino acid oxidase. Wild type D-amino acid oxidase with catalytic activity on a catalytic substrate DL-glufosinate-ammonium is obtained through screening, and the activity and soluble expression of the D-amino acid oxidase obtained through host expression are further improved through methods of screening expression hosts, adding molecular chaperones and the like. The method for preparing alpha-keto acid by using D-amino acid oxidase provided by the invention realizes kinetic resolution of racemic glufosinate-ammonium, and can be widely applied to production and application of L-glufosinate-ammonium.
本发明涉及生物化工技术领域，具体涉及一种表达D-氨基酸氧化酶的方法及其应用。本发明通过筛选获得了对催化底物DL-草铵膦具有催化活力的野生型D-氨基酸氧化酶，并通过对表达宿主的筛选以及添加分子伴侣等方法，使得宿主表达获得的D-氨基酸氧化酶的活性和可溶性表达进一步提高。利用本发明提供的D-氨基酸氧化酶制备α-酮酸的方法实现了外消旋草铵膦的动力学拆分，能够广泛应用到L-草铵膦的生产应用中。 |
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本发明涉及生物化工技术领域，具体涉及一种表达D-氨基酸氧化酶的方法及其应用。本发明通过筛选获得了对催化底物DL-草铵膦具有催化活力的野生型D-氨基酸氧化酶，并通过对表达宿主的筛选以及添加分子伴侣等方法，使得宿主表达获得的D-氨基酸氧化酶的活性和可溶性表达进一步提高。利用本发明提供的D-氨基酸氧化酶制备α-酮酸的方法实现了外消旋草铵膦的动力学拆分，能够广泛应用到L-草铵膦的生产应用中。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230704&DB=EPODOC&CC=CN&NR=116376940A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76418</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230704&DB=EPODOC&CC=CN&NR=116376940A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>XU JIAQI</creatorcontrib><creatorcontrib>LOU YIYUAN</creatorcontrib><creatorcontrib>WANG ZIYUAN</creatorcontrib><creatorcontrib>ZHOU SHUGUANG</creatorcontrib><creatorcontrib>YANG LIRONG</creatorcontrib><creatorcontrib>YU SHENLUAN</creatorcontrib><creatorcontrib>ZHANG ZEWEI</creatorcontrib><creatorcontrib>JIN YONG</creatorcontrib><creatorcontrib>ZHOU HAISHENG</creatorcontrib><creatorcontrib>KANG XIN</creatorcontrib><title>Method for expressing D-amino acid oxidase and application of D-amino acid oxidase</title><description>The invention relates to the technical field of biochemical engineering, in particular to a method for expressing D-amino acid oxidase and application of the D-amino acid oxidase. Wild type D-amino acid oxidase with catalytic activity on a catalytic substrate DL-glufosinate-ammonium is obtained through screening, and the activity and soluble expression of the D-amino acid oxidase obtained through host expression are further improved through methods of screening expression hosts, adding molecular chaperones and the like. The method for preparing alpha-keto acid by using D-amino acid oxidase provided by the invention realizes kinetic resolution of racemic glufosinate-ammonium, and can be widely applied to production and application of L-glufosinate-ammonium.
本发明涉及生物化工技术领域，具体涉及一种表达D-氨基酸氧化酶的方法及其应用。本发明通过筛选获得了对催化底物DL-草铵膦具有催化活力的野生型D-氨基酸氧化酶，并通过对表达宿主的筛选以及添加分子伴侣等方法，使得宿主表达获得的D-氨基酸氧化酶的活性和可溶性表达进一步提高。利用本发明提供的D-氨基酸氧化酶制备α-酮酸的方法实现了外消旋草铵膦的动力学拆分，能够广泛应用到L-草铵膦的生产应用中。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZAjyTS3JyE9RSMsvUkitKChKLS7OzEtXcNFNzM3My1dITM5MUcivyExJLE5VSMxLUUgsKMjJTE4syczPU8hPw6qOh4E1LTGnOJUXSnMzKLq5hjh76KYW5MenFhckJqfmpZbEO_sZGpoZm5tZmhg4GhOjBgCsdTc1</recordid><startdate>20230704</startdate><enddate>20230704</enddate><creator>XU JIAQI</creator><creator>LOU YIYUAN</creator><creator>WANG ZIYUAN</creator><creator>ZHOU SHUGUANG</creator><creator>YANG LIRONG</creator><creator>YU SHENLUAN</creator><creator>ZHANG ZEWEI</creator><creator>JIN YONG</creator><creator>ZHOU HAISHENG</creator><creator>KANG XIN</creator><scope>EVB</scope></search><sort><creationdate>20230704</creationdate><title>Method for expressing D-amino acid oxidase and application of D-amino acid oxidase</title><author>XU JIAQI ; LOU YIYUAN ; WANG ZIYUAN ; ZHOU SHUGUANG ; YANG LIRONG ; YU SHENLUAN ; ZHANG ZEWEI ; JIN YONG ; ZHOU HAISHENG ; KANG XIN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116376940A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>XU JIAQI</creatorcontrib><creatorcontrib>LOU YIYUAN</creatorcontrib><creatorcontrib>WANG ZIYUAN</creatorcontrib><creatorcontrib>ZHOU SHUGUANG</creatorcontrib><creatorcontrib>YANG LIRONG</creatorcontrib><creatorcontrib>YU SHENLUAN</creatorcontrib><creatorcontrib>ZHANG ZEWEI</creatorcontrib><creatorcontrib>JIN YONG</creatorcontrib><creatorcontrib>ZHOU HAISHENG</creatorcontrib><creatorcontrib>KANG XIN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>XU JIAQI</au><au>LOU YIYUAN</au><au>WANG ZIYUAN</au><au>ZHOU SHUGUANG</au><au>YANG LIRONG</au><au>YU SHENLUAN</au><au>ZHANG ZEWEI</au><au>JIN YONG</au><au>ZHOU HAISHENG</au><au>KANG XIN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for expressing D-amino acid oxidase and application of D-amino acid oxidase</title><date>2023-07-04</date><risdate>2023</risdate><abstract>The invention relates to the technical field of biochemical engineering, in particular to a method for expressing D-amino acid oxidase and application of the D-amino acid oxidase. Wild type D-amino acid oxidase with catalytic activity on a catalytic substrate DL-glufosinate-ammonium is obtained through screening, and the activity and soluble expression of the D-amino acid oxidase obtained through host expression are further improved through methods of screening expression hosts, adding molecular chaperones and the like. The method for preparing alpha-keto acid by using D-amino acid oxidase provided by the invention realizes kinetic resolution of racemic glufosinate-ammonium, and can be widely applied to production and application of L-glufosinate-ammonium.
本发明涉及生物化工技术领域，具体涉及一种表达D-氨基酸氧化酶的方法及其应用。本发明通过筛选获得了对催化底物DL-草铵膦具有催化活力的野生型D-氨基酸氧化酶，并通过对表达宿主的筛选以及添加分子伴侣等方法，使得宿主表达获得的D-氨基酸氧化酶的活性和可溶性表达进一步提高。利用本发明提供的D-氨基酸氧化酶制备α-酮酸的方法实现了外消旋草铵膦的动力学拆分，能够广泛应用到L-草铵膦的生产应用中。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for expressing D-amino acid oxidase and application of D-amino acid oxidase |
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