Method for expressing D-amino acid oxidase and application of D-amino acid oxidase

The invention relates to the technical field of biochemical engineering, in particular to a method for expressing D-amino acid oxidase and application of the D-amino acid oxidase. Wild type D-amino acid oxidase with catalytic activity on a catalytic substrate DL-glufosinate-ammonium is obtained thro...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	XU JIAQI, LOU YIYUAN, WANG ZIYUAN, ZHOU SHUGUANG, YANG LIRONG, YU SHENLUAN, ZHANG ZEWEI, JIN YONG, ZHOU HAISHENG, KANG XIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention relates to the technical field of biochemical engineering, in particular to a method for expressing D-amino acid oxidase and application of the D-amino acid oxidase. Wild type D-amino acid oxidase with catalytic activity on a catalytic substrate DL-glufosinate-ammonium is obtained through screening, and the activity and soluble expression of the D-amino acid oxidase obtained through host expression are further improved through methods of screening expression hosts, adding molecular chaperones and the like. The method for preparing alpha-keto acid by using D-amino acid oxidase provided by the invention realizes kinetic resolution of racemic glufosinate-ammonium, and can be widely applied to production and application of L-glufosinate-ammonium. 本发明涉及生物化工技术领域，具体涉及一种表达D-氨基酸氧化酶的方法及其应用。本发明通过筛选获得了对催化底物DL-草铵膦具有催化活力的野生型D-氨基酸氧化酶，并通过对表达宿主的筛选以及添加分子伴侣等方法，使得宿主表达获得的D-氨基酸氧化酶的活性和可溶性表达进一步提高。利用本发明提供的D-氨基酸氧化酶制备α-酮酸的方法实现了外消旋草铵膦的动力学拆分，能够广泛应用到L-草铵膦的生产应用中。