RdCHS4 protein related to rhododendron delavayi anthocyanin metabolism, recombinant vector and application of RdCHS4 protein

The invention belongs to the technical field of bioengineering, and particularly relates to RdCHS4 protein related to rhododendron delavayi anthocyanin metabolism, a recombinant vector and application of the RdCHS4 protein. The invention provides an RdCHS4 protein related to rhododendron delavayi anthocyanin metabolism. The amino acid sequence of the RdCHS4 protein is as shown in SEQ ID NO: 4. Results of embodiments show that the RdCHS4 protein provided by the invention can restore cotyledons and hypocotyls of arabidopsis CHS mutant seedlings to purple, and petals of transgenic tobacco plants are deepened in color. Therefore, the RdCHS4 protein provided by the invention can promote the formation of plant anthocyanin. 本发明属于生物工程技术领域，具体涉及一种与马缨杜鹃花色苷代谢相关的RdCHS4蛋白、重组载体及其应用。本发明提供了一种与马缨杜鹃花色苷代谢相关的RdCHS4蛋白，所述RdCHS4蛋白的氨基酸序列如SEQ ID NO:4所示。实施例结果表明：本发明提供的RdCHS4蛋白可使拟南芥CHS突变体幼苗子叶及下胚轴恢复为紫色，转基因烟草植株花瓣颜色加深。可见，本发明提供的RdCHS4蛋白可以促进植株花色苷的形成。