Preparation method of umbilical cord blood hematopoietic stem cells

The invention relates to the technical field of biology, in particular to a preparation method of umbilical cord blood hematopoietic stem cells. The preparation method of the umbilical cord blood hematopoietic stem cells comprises the step of separating umbilical cord blood by using a stem cell separating medium, wherein the stem cell separating medium comprises sodium diatrizoate, tea saponin, hydroxyethyl cellulose, polysucrose and disodium hydrogen phosphate. The umbilical cord blood hematopoietic stem cells prepared from the stem cell separating medium are high in motility rate, high in purity, high in proliferation speed, free of toxic and side effects on human bodies, safe and reliable. 本发明涉及生物技术领域，具体涉及一种脐带血造血干细胞的制备方法。所述的脐带血造血干细胞的制备方法包括使用干细胞分离液对脐带血进行分离，所述干细胞分离液包括泛影酸钠、茶皂素、羟乙基纤维素、聚蔗糖和磷酸氢二钠。本发明使用该干细胞分离液制备得到的脐带血造血干细胞的活率高，纯度高，增殖速度较快，对人体无毒副作用，安全可靠。