Construction method of zebra fish enteritis model
The invention belongs to the field of animal models, and particularly discloses a construction method of a zebrafish enteritis model. Sleep insufficiency caused by illumination relates to the field of animal models, active oxygen is continuously accumulated in intestinal tracts, oxidative damage is...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention belongs to the field of animal models, and particularly discloses a construction method of a zebrafish enteritis model. Sleep insufficiency caused by illumination relates to the field of animal models, active oxygen is continuously accumulated in intestinal tracts, oxidative damage is caused to the intestinal tracts, the barrier function of the intestinal tracts is destroyed, and finally intestinal cell apoptosis is caused. Meanwhile, after the fructose enters the intestinal tract, the fructose is decomposed by fructokinase, endoplasmic reticulum stress and intestinal tract inflammation are caused, the intestinal tract barrier is damaged, and the death rate of the zebra fish can be increased due to the high-concentration fructose. Therefore, according to the method, illumination is adopted for sleep deprivation to increase accumulation of intestinal active oxygen, and meanwhile, a low-concentration fructose solution is combined for treatment to construct the zebrafish enteritis model. The method |
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