Fluorescent microscopic imaging system and method for detecting circulating tumor cells

The invention relates to the technical field of fluorescence microscopic imaging, and particularly discloses a fluorescence microscopic imaging system and method for detecting circulating tumor cells, and the method comprises the following steps: firstly, obtaining a fluorescence development image o...

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Hauptverfasser: ZHANG KAISHAN, LI CHAO, RAO LANGQING, LIU YANSHENG, ZHAO DAN, YU LEI, GUO ZHIMIN, MA NING, TIAN HUA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of fluorescence microscopic imaging, and particularly discloses a fluorescence microscopic imaging system and method for detecting circulating tumor cells, and the method comprises the following steps: firstly, obtaining a fluorescence development image of a detected sample; the method comprises the following steps: firstly, carrying out image preprocessing on a fluorescent development image, and obtaining a fluorescent shallow layer feature map through a shallow layer feature extractor based on a first convolutional neural network model; the fluorescent shallow layer feature map passes through a spatial attention module and a deep feature extractor based on a second convolutional neural network model to obtain a fluorescent deep layer feature map, and finally, after the spatial enhanced fluorescent shallow layer feature map and the fluorescent deep layer feature map are fused, image semantic segmentation is carried out through a decoder to obtain a segmentation re