SgRNA targeting human DMD site and application thereof

The invention discloses sgRNA targeting a human DMD site and application of the sgRNA, and belongs to the technical field of gene editing and cell engineering. The target site of the sgRNA is located in the 50th exon region of the human DMD gene; the nucleotide sequence of the sgRNA is as shown in SEQ ID NO. 1. The DMD gene in the HEK293T cell is subjected to gene editing by using the ABE and CBE systems in the single-base editing system, the operation is simple, and the editing efficiency is high. 本发明公开了一种靶向人DMD位点的sgRNA及其应用，属于基因编辑和细胞工程技术领域。本发明的sgRNA的靶向位点位于人DMD基因的第50号外显子区域；所述sgRNA的核苷酸序列如SEQ ID NO.1所示。本发明利用单碱基编辑系统中的ABE和CBE系统对HEK293T细胞中的DMD基因进行基因编辑，操作简单，编辑效率高。